px330 bbsi pitch addgene 127875 121 (Addgene inc)
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px330 bbsi pitch addgene 127875 121
Px330 Bbsi Pitch Addgene 127875 121, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px330+bbsi+pitch/pX330-BbsI-PITCh+(Plasmid+%23127875)/pm41904131-261-40-41
Average 93 stars, based on 68 article reviews
Px330 Bbsi Pitch Addgene 127875 121, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/px330+bbsi+pitch/pX330-BbsI-PITCh+(Plasmid+%23127875)/pm41904131-261-40-41
Average 93 stars, based on 68 article reviews
px330 bbsi pitch addgene 127875 121 - by Bioz Stars,
2026-09
93/100 stars
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Plasmid Preparation:Article Title: SMG1:SMG8:SMG9-complex integrity maintains robustness of nonsense-mediated mRNA decay Article Snippet: .. The Article Title: Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated transcriptome in human cells Article Snippet: .. For FKBP12F36V-tagging of UPF1, the knock-ins were performed using the CRIS-PITCh v2 system consisting of a Article Title: Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome. Article Snippet: .. Knock-in cells using CRIS-PITCh v2 For FKBP12F36V-tagging of UPF1, the knock-ins were performed using the CRIS-PITCh v2 system189 consisting of a pX330-BbsIPITCh encoding the gene specific sgRNA (5′-CCCGTACGCCTCCACGCTCA-3′) and the pCRIS-PITChv2-PurR-FKBP (UPF1 N) donor plasmid (based on Addgene #91796). Article Title: SMG1:SMG8:SMG9-complex integrity supports efficient execution of nonsense-mediated mRNA decay Article Snippet: .. The plasmid pX330-PITCh_SMG8 is based on Article Title: Zinc-finger proteins with a co-opted capsid domain anchor nucleosomes over transposon sequences Article Snippet: To establish a HAP1 cell line with its endogenous ZNF24 gene tagged with an FKBP12 F36V degron, the cells were first sorted based on cellular sizes with FACSAria II (BD Biosciences) to enrich for haploid cells as previously described ( ). .. The repair template plasmid construction and sgRNA cloning were performed as previously described ( , ). pCRIS-PITChv2-BSD-dTAG (BRD4) was a gift from James Bradner & Behnam Nabet (Addgene plasmid # 91792 ; http://n2t.net/addgene:91792 ; RRID:Addgene_91792) and Knock-In:Article Title: Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome. Article Snippet: .. Knock-in cells using CRIS-PITCh v2 For FKBP12F36V-tagging of UPF1, the knock-ins were performed using the CRIS-PITCh v2 system189 consisting of a pX330-BbsIPITCh encoding the gene specific sgRNA (5′-CCCGTACGCCTCCACGCTCA-3′) and the pCRIS-PITChv2-PurR-FKBP (UPF1 N) donor plasmid (based on Addgene #91796). Generated:Article Title: Loss of CFIm activates YAP/TAZ and connects mRNA cleavage and polyadenylation inhibition to BRCAness Article Snippet: .. PITCh dTAG donor vectors for NUDT21 targeting were generated by insertion of custom guide RNA sequences using the following plasmids: Cloning:Article Title: Zinc-finger proteins with a co-opted capsid domain anchor nucleosomes over transposon sequences Article Snippet: To establish a HAP1 cell line with its endogenous ZNF24 gene tagged with an FKBP12 F36V degron, the cells were first sorted based on cellular sizes with FACSAria II (BD Biosciences) to enrich for haploid cells as previously described ( ). .. The repair template plasmid construction and sgRNA cloning were performed as previously described ( , ). pCRIS-PITChv2-BSD-dTAG (BRD4) was a gift from James Bradner & Behnam Nabet (Addgene plasmid # 91792 ; http://n2t.net/addgene:91792 ; RRID:Addgene_91792) and |